DDB1 (human) (rec.) (His) (Avi) (Biotin)

DNA Damage-binding Protein 1; DDB p127 Subunit; DNA Damage-binding Protein a (DDBa); HBV X-associated Protein 1 (XAP-1); UV-damaged DNA-binding Factor

Lot dependent.

32160000

liquid

Liquid. In 25mM HEPES pH 7.5, 150mM NaCl, 0.1mM TCEP (no detergents or glycerol).

Aliquot to avoid freeze/thaw cycles.

Recombinant Protein. Recombinant human DDB1 (aa 1-1140) with a N-terminal 8x-His Tag followed by an Avi-Tag that was endogenous biotinylated. Produced in Sf21 cells. Liquid. In 25mM HEPES pH 7.5, 150mM NaCl, 0.1mM TCEP (no detergents or glycerol). DNA damage-binding protein 1, which is involved in both DNA repair and protein ubiquitination, is part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively. Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruits proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Functions as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. The protein has an N-terminal 8x-His Tag followed by an Avi-Tag that was endogenously biotinylated during expression in Sf21 Cells.

Q16531

Plastic Vial

DNA damage-binding protein 1, which is involved in both DNA repair and protein ubiquitination, is part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively. Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruits proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Functions as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. The protein has an N-terminal 8x-His Tag followed by an Avi-Tag that was endogenously biotinylated during expression in Sf21 Cells.

>98% (SDS-PAGE)

Recombinant human DDB1 (aa 1-1140) with a N-terminal 8x-His Tag followed by an Avi-Tag that was endogenous biotinylated.

Sf21 cells

His

Non-hazardous

Other Proteins

12352202

Stable for at least 1 year after receipt when stored at -80°C.