Elabscience

Purified Anti-Whitlow 218 Linker Antibody[DA011]

Product Code:
 
AN009990P
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
DA011
Regulatory Status:
 
RUO
Application:
 
Flow Cytometry
Shipping:
 
Ice bag
Storage:
 
Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles.
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HEK293T cells transfected with anti-CD19(FMC63 scFv with Whitlow/218 Linker) CAR were stained with 0.2 ug Purified Anti-Whitlow/218 Linker Antibody[DA011] (Right) and 0.2 ug Rabbit IgG, κ Isotype Control (Left), followed by APC-conjugated Goat Anti-Rabbit IgG Secondary Antibody.
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HEK293T cells transfected with anti-CD19(FMC63 scFv with Whitlow/218 Linker) CAR were stained with 0.2 ug Purified Anti-Whitlow/218 Linker Antibody[DA011] (Right) and 0.2 ug Rabbit IgG, κ Isotype Control (Left), followed by APC-conjugated Goat Anti-Rabbit IgG Secondary Antibody.

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CodeSizePrice
AN009990P-25ug25ug£85.00
Quantity:
AN009990P-100ug100ug£116.00
Quantity:
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Background:
The whitlow/218 linker, with the sequence GSTSGSGKPGSGEGSTKG, is a commonly used synthetic peptide linker. Whitlow/218 linker often leveraged to connect the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The scFv containing the 218 linker showed reduced aggregation and was found to be more stable to proteolysis in vitro.
Buffer:
Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling.
Concentration:
≥ 1 mg/mL
Conjugation:
Unconjugated
Dilution:
FCM 2 ug/mL(0.5x106-1x106 cells)
Expiry date:
12 months
Immunogen:
Synthetic peptide
Purification Method:
>98%, Protein A/G purified

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