Elabscience

Purified Anti-Human/Monkey CD16 Antibody[DA002]

Product Code:
 
AN010980P
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, κ
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
DA002
Regulatory Status:
 
RUO
Target Species:
  • Cynomolgus Monkey
  • Human
  • Rhesus Macaque
Applications:
  • Blocking
  • Flow Cytometry
Shipping:
 
Ice bag
Storage:
 
Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles.
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Human peripheral blood lymphocytes were stained with 0.2 ug Purified Anti-Human CD16 Antibody[3G8] (Right) and 0.2 ug Mouse IgG1, κ Isotype Control (Left), followed by APC-conjugated Goat Anti-Mouse IgG Secondary Antibody, then anti-Human CD56 PE-conjugated Monoclonal Antibody.

Human peripheral blood lymphocytes were stained with 0.2 ug Purified Anti-Human CD16 Antibody[3G8] (Right) and 0.2 ug Mouse IgG1, κ Isotype Control (Left), followed by APC-conjugated Goat Anti-Mouse IgG Secondary Antibody, then anti-Human CD56 PE-conjugated Monoclonal Antibody.

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CodeSizePrice
AN010980P-25ug25ug£73.00
Quantity:
AN010980P-100ug100ug£92.00
Quantity:
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Background:
CD16 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD16 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD16 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD16 appears to be expressed later in the immune response than CD86. CD16 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.
Buffer:
Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling.
Concentration:
≥ 1 mg/mL
Conjugation:
Unconjugated
Dilution:
FCM 2 ug/mL(0.5x106-1x106 cells)
Expiry date:
12 months
GeneID:
22142215
Immunogen:
Recombinant Human CD16 protein
Purification Method:
>98%, Protein A/G purified
Target Synonym:
Fc gamma receptor, Fc gamma receptor 3, FcγRIII
UNIProt ID:
P08637, O75015,
Usage:
Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ug per 106 cells in 100 uL volume or 100 uL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

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