Elabscience

Purified Anti-G4S linker Antibody[DA010]

Product Code:
 
AN011300P
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
DA010
Regulatory Status:
 
RUO
Application:
 
Flow Cytometry
Shipping:
 
Ice bag
Storage:
 
Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles.
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HEK293T cells transfected with anti-CD19(FMC63 scFv with G4S linker) CAR were stained with 0.2 ug Purified Anti- G4S linker Antibody[DA010] (Right) and 0.2 ug Rabbit IgG, κ Isotype Control (Left), followed by APC-conjugated Goat Anti-Rabbit IgG Secondary Antibody.
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HEK293T cells transfected with anti-CD19(FMC63 scFv with G4S linker) CAR were stained with 0.2 ug Purified Anti- G4S linker Antibody[DA010] (Right) and 0.2 ug Rabbit IgG, κ Isotype Control (Left), followed by APC-conjugated Goat Anti-Rabbit IgG Secondary Antibody.

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CodeSizePrice
AN011300P-25ug25ug£85.00
Quantity:
AN011300P-100ug100ug£116.00
Quantity:
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Background:
The G4S linker is emerged as the most common linking peptides for scFvs. It is composed of the repeated sequence (Gly-Gly-Gly-Gly-Ser) and commonly found as either a 15-mer (G4S)3 or 20-mer(G4S)4 within scFv fragments. The G4S linker has become an integral part of the vast majority of CARs as it links the VH and VL recognition domains of scFvs. The G4S linker is commonly used in CARs targeting solid tumor antigens, such as HER2 and CEA, while the whitlow/218 linker peptide is predominantly used in CAR constructs targeting hematological malignancies such as CD19 CAR products.
Buffer:
Phosphate-buffered solution, pH 7.2, containing 0.05% non-protein stabilizer. Dialyze to completely remove the stabilizer prior to labeling.
Concentration:
≥ 1 mg/mL
Conjugation:
Unconjugated
Dilution:
FCM 2 ug/mL(0.5x106-1x106 cells)
Expiry date:
12 months
Immunogen:
Synthetic peptide
Purification Method:
>98%, Protein A/G purified

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