Anti-SUMO Tag Antibody Sepharose Purification Kit

Affinity purified anti-SUMO antibodies, developed in mice, were conjugated to NHS-sepharose. It is an efficient technique for isolating recombinant proteins or mammalian expression proteins. The SUMO epitope system relies on an approximately 100 amino acids long recombinant antibody, which is able to react with N- and C- terminal SUMO-tagged fusion proteins and may be used for immunoprecipitation or immune affinity purification. The purified antibody is immobilized at 3-5 mg antibody per mL of 50% slurry, and this kit allows a rapid and efficient affinity purification of active SUMO fusion proteins. The affinity resin allows an efficient binding of SUMO fusion proteins without the need for preliminary steps and calibrations. The affinity bound SUMO fusion proteins can be efficiently eluted from the resin by acid condition. The eluted proteins can be used for characteristic analysis.