Sciencell

GeneQuery™ Human B and T Cells Development Markers qPCR Array Kit

Product Code:
 
SC-GK116
Product Group:
 
PCR Kits
Supplier:
 
Sciencell
Regulatory Status:
 
RUO
Target Species:
 
Human
Shipping:
 
Ambient
Storage:
 
4°C
1 / 1
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Further Information

Description:
ScienCell's GeneQuery™ Human B and T Cells Development Markers qPCR Array Kit (GQH-BTD) is designed to facilitate gene expression profiling of 40 marker genes involved in the differentiation of common lymphoid progenitors (CLPs) into B and T cells. Brief examples of how included genes may be grouped according to their functions are shown below:
  • CLP markers: - MME, CD34, IL7R, FLT3
  • ProB-PreB-immature B cell markers: - MME, MS4A1, SPN, PTPRC, IL7R, CD79A/B, CD40, CD19
  • B1 cell markers: - CD19, MS4A1, CD27, SPN, CD40LG
  • Regulatory B cell markers: - CD1D, CD5, CD19, CR2, CD24
  • Marginal Zone B cell markers: - CD1C, CD19, MS4A1, CR2, CD24
  • Follicular B cell markers: - CD19, MS4A1, CR2, CD22, FCER2
  • Memory B cell markers: - CD27, CD80, CD84, CD86
  • Plasmablast-plasma cell markers: - CD19, CD27, CD38, IL6R, SDC1, SLAMF7
  • Double-negative and double-positive stage T cell markers: - CD1A, CD3D/E/G, CD4, CD5, CD7, CD8A/B, CD38, SELE/L/P, CD160, CD200
      • Note : - all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC). GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg^2+ , and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
Extra Description:
This kit is designed to facilitate gene expression profiling of 40 marker genes involved in the differentiation of common lymphoid progenitors (CLPs) into B and T cells.