Our supplier, abm, is proud to offer an expanded line of CRISPR-related products and services, including a genome-wide collection of CRISPR Cas9 sgRNA-expressing vectors and viruses for efficient gene knockout experiments. Each human, mouse, or rat gene is targeted with a set of three sgRNAs, available across Lentiviral, AAV, and non-viral formats.
Depending on your project requirements, the sgRNA may be delivered together with Cas9 in a single plasmid (All-in-One Vector) or separately in a two-plasmid (Dual Vector) format.
Read the guide below to help you choose the right format for your research!
Lentiviral
AAV
Non-viral
Integrate into the genome, providing stable Cas9/sgRNA expression.
Deliver episomal DNA, but have a limited packaging capacity (~4.7 kb). abm’s vectors use the smaller saCas9 variant to fit within this size limit.
Provide transient expression, reducing off-target effects. Non-viral methods include: Plasmid transfection (Cas9 + sgRNA), mRNA delivery (Cas9 mRNA + synthetic sgRNA), RNP complexes (Cas9 protein pre-complexed with sgRNA)
Ideal for:
Ideal for:
Ideal for:
- Stable, long-term knockout
- Working with hard-to-transfect (e.g., suspension cells, primary T cells), stem cells or dividing cell lines
- Generating pooled or arrayed CRISPR knockout libraries for functional genomics and drug screening
- In vivo knockout studies due to their low immunogenicity and tissue-specific tropism
- Targeting post-mitotic cells such as neurons and muscle cells
- Short-term functional assays
- Rapid, transient knockouts with minimal genomic integration risk
All-in-One Lentiviral Vectors
An All-in-One vector system offers easy, one-step transfection with increased transfection efficiency and 1:1 sgRNA and Cas9 expression.
abm’s All-in-One Lentiviral Vector is a leading tool for CRISPR gene knockout, enabling sgRNA expression from the U6 promoter and expression of Cas9 and Puromycin (can be modified to other markers) selection from the SFFV promoter.
This single-vector system streamlines experiments by leveraging lentiviral delivery across diverse cell types and requiring only one construct to achieve precise gene knockout.
abm’s All-in-One Lentiviral Vector Sets have been successfully utilised for gene knockout by researchers around the world and have been published in high-impact journals such as Nature Communications and Nature Microbiology. Together, these features make abm’s CRISPR Cas9 tools a robust and versatile solution for streamlined, high-confidence gene knockout workflows.
Dual Vector System
A Dual Vector system is suitable for multiplex experiments, as a pool of several different sgRNA vectors can be transfected alongside Cas9-expressing constructs.
abm’s Dual Vector system comprises a Cas9-expressing construct and a separate construct carrying the sgRNA (driven by the U6 promoter) as well as the Neomycin resistance (controlled by a PGK promoter)
for downstream screening.
A multiplex sgRNA CRISPR vector is also available, which allows for the expression of up to 4 sgRNAs from a single construct.
Information provided by abm.
Caltag is the distributor of abm products in the UK and Ireland. If you have any questions about these products, please contact us.