High Quality Green Fluorescent Protein (GFP) Antibodies

High Quality Green Fluorescent Protein (GFP) Antibodies

Nordic-MUbio have recently released a new range of products directed against the native form of GFP. Nordic’s anti-GFP antibodies bring a number of advantages over antibodies produced against recombinant, or variant forms of GFP.

This includes the ability to detect GFP produced from a wide range of different sources, including native, recombinant, and a number of different mutants, without suffering from high levels of non-specific binding/background.

Part of the reason many anti-recombinant GFP antibodies suffer from high background issues is due to contamination of an innoculum with other E.coli components, leading to increased non-specific binding.

Nordic’s products were tested for cross-reactivity to a wide range of antigens, including many derived from E.coli, and demonstrated no cross-reactivity.

Available Products:

Rabbit anti-GFP (native) – Ammonium Sulfate fraction (0.1 mg)

Rabbit anti-GFP (native) – Protein A Purified (0.1 mg)

Rabbit anti-GFP (native) – Affinity Purified (0.1 mg)

Rabbit anti-GFP (native):PO – Soyabean Peroxidase Conjugate (0.1 ml)

Chicken anti-GFP (native) – Ammonium Sulfate fraction (0.1 mg)

Recombinant Green Fluorescent Protein (wild type) – Recombinant Protein (0.1 mg) 

To ensure Nordic-MUbio’s new GFP antibody range is of the highest quality, a number of comparison experiments were run with the bestselling antibodies from other suppliers against recombinant GFP,
assessing purity, specificity, and titre of the antibodies:

nordic_GFP

Example Size Exclusion Chromatography (HPLC) Results:GFP_Nordic

The HPLC analyses of the Nordic-MUbio affinity purified antibodies show just a single peak, illustrating their purity. The competitor antibodies with multiple peaks have contaminants, such as serum albumin, which can affect the results of e.g. Western Blots or ELISAs.

Nordic-GFP

Background Information

Green Fluorescent Protein (GFP) is a 27kD protein derived from the Aequorea victoria (A.victoria) jellyfish. It is a naturally fluorescent protein which has revolutionised life science and has been used to study proteins in a wide range of applications. GFP has been used extensively to monitor gene expression; to localise proteins at the cellular and sub-cellular level; to track metastatic cells and as a tag to identify proteins in vitro.

 

A. victoria is a bioluminescent jellyfish, or hydromedusa, found off the west coast of North America.

The enormous interest in GFP as a protein marker has given rise to the development of polyclonal and monoclonal antibodies against GFP, used for the immunolocalisation and immunoblotting of GFPfused proteins in a variety of applications.

Since its discovery, many variants of GFP have been engineered making antibodies recognising a wide range of these variants an important and useful scientific tool for use alongside GFP-fused proteins.

Nordic-MUbio’s anti-GFP antibodies are distributed in the UK & ROI by Caltag Medsystems. For more information about this range, or any other products available from Nordic-NUbio, please contact us and we will be happy to help.

 

High Quality Green Fluorescent Protein (GFP) Antibodies