Nordic-MUbio have recently released a new range of products directed against the native form of GFP. Nordic’s anti-GFP antibodies bring a number of advantages over antibodies produced against recombinant, or variant forms of GFP.
This includes the ability to detect GFP produced from a wide range of different sources, including native, recombinant, and a number of different mutants, without suffering from high levels of non-specific binding/background.
Part of the reason many anti-recombinant GFP antibodies suffer from high background issues is due to contamination of an innoculum with other E.coli components, leading to increased non-specific binding.
Nordic’s products were tested for cross-reactivity to a wide range of antigens, including many derived from E.coli, and demonstrated no cross-reactivity.
Available Products:
Rabbit anti-GFP (native) – Ammonium Sulfate fraction (0.1 mg)
Rabbit anti-GFP (native) – Protein A Purified (0.1 mg)
Rabbit anti-GFP (native) – Affinity Purified (0.1 mg)
Rabbit anti-GFP (native):PO – Soyabean Peroxidase Conjugate (0.1 ml)
Chicken anti-GFP (native) – Ammonium Sulfate fraction (0.1 mg)
Recombinant Green Fluorescent Protein (wild type) – Recombinant Protein (0.1 mg)
To ensure Nordic-MUbio’s new GFP antibody range is of the highest quality, a number of comparison experiments were run with the bestselling antibodies from other suppliers against recombinant GFP,
assessing purity, specificity, and titre of the antibodies:
Example Size Exclusion Chromatography (HPLC) Results:
The HPLC analyses of the Nordic-MUbio affinity purified antibodies show just a single peak, illustrating their purity. The competitor antibodies with multiple peaks have contaminants, such as serum albumin, which can affect the results of e.g. Western Blots or ELISAs.
Background Information
Green Fluorescent Protein (GFP) is a 27kD protein derived from the Aequorea victoria (A.victoria) jellyfish. It is a naturally fluorescent protein which has revolutionised life science and has been used to study proteins in a wide range of applications. GFP has been used extensively to monitor gene expression; to localise proteins at the cellular and sub-cellular level; to track metastatic cells and as a tag to identify proteins in vitro.
A. victoria is a bioluminescent jellyfish, or hydromedusa, found off the west coast of North America.
The enormous interest in GFP as a protein marker has given rise to the development of polyclonal and monoclonal antibodies against GFP, used for the immunolocalisation and immunoblotting of GFPfused proteins in a variety of applications.
Since its discovery, many variants of GFP have been engineered making antibodies recognising a wide range of these variants an important and useful scientific tool for use alongside GFP-fused proteins.
Nordic-MUbio’s anti-GFP antibodies are distributed in the UK & ROI by Caltag Medsystems. For more information about this range, or any other products available from Nordic-NUbio, please contact us and we will be happy to help.