Mechanisms and detection methods of p62/SQSTM1 and its importance in the autophagy pathway.

Mechanisms and detection methods of p62/SQSTM1 and its importance in the autophagy pathway.

MBL International, distributed by Caltag MedsystemsMechanisms of Autophagy

The ubiquitin-conjugating protein p62/SQSTM1 is thought to be a scaffold protein that interacts with a variety of molecules involved in toll-like receptor (TLR) signaling, such as TRAF6, ERK, and aPKC. Recent evidence suggests that p62 binds directly to the autophagosome marker LC3 and is then selectively degraded by autophagy.

p62 contains multiple phosphorylation sites. Sequential phosphorylation of these sites regulates biological defence mechanisms such as selective autophagy and the Keap1-Nrf2 system. Therefore, the failure of the p62 pathway is associated with various diseases such as cancer and many neurodegenerative diseases.


To learn more about the mechanisms of p62, please refer to MBL’s whitepaper ” “p62/SQSTM1: The molecule that links autophagy to the Keap1-Nrf2 system“, written by Drs. Masaaki Komatsu and Yoshinobu Ichimura, Niigata University. They are leading researchers in Autophagy, especially p62 and Keap1-Nrf2 interaction. Dr. Komatsu was recognized as one of the most highly cited researchers in Autophagy in 2014 and 2015 by Clarivate Analytics.

Download White Paper

p62 Detection

How do you detect p62 or phospho-p62 using antibodies? Western blotting? Immunohistochemistry? Quantification of biomarkers is essential for medical and diagnostic application. Selective autophagy and Keap1-Nrf2 are already being discussed for their use as a diagnostic as well as other medical applications.

MBL International has developed new sandwich ELISA kits that can semi-quantify total p62 and its phosphorylation level at Ser403 and Ser349. Phospho-p62 ELISA kits are the first kits to quantify these markers by ELISA in the world research market. It is a chance to get a new discovery in the Autophagy area!

CY-7055: CycLex® Total p62 ELISA Kit
CY-7056: CycLex® Phospho-p62 Ser349 ELISA Kit
CY-7057: CycLex® Phospho-p62 Ser403 ELISA Kit

These kits can help you to prepare cell lysate easily including cell lysis buffers. Phosphatase inhibitor solution is also included in the phosho-p62 kits. They can be used for human and mouse samples.

Total p62 and phospho-p62 levels in HeLa cells
Total p62 and phospho-p62 levels in HeLa cells


MBL also offer antibodies against p62 and phopho-p62. MBLI offers highly cited polyclonal and monoclonal p62 antibodies that can be used for multiple applications. Fluorescent dyes or HRP conjugated antibodies are also offered. Discover your perfect one from the following list:

Code #Product NameCloneHost SpeciesCross-reactivityApplicationConjugate
M162-3Anti-p62 (SQSTM1) (Human) mAb5F2MouseHuWB, IP, IF, IHC, FCM
M162-A48Anti-p62 (SQSTM1) (Human) mAb-Alexa Fluor® 4885F2MouseHuIF, FCMAlexa Fluor® 488
M162-A59Anti-p62 (SQSTM1) (Human) mAb-Alexa Fluor® 5945F2MouseHuIFAlexa Fluor® 594
M162-A64Anti-p62 (SQSTM1) (Human) mAb-Alexa Fluor® 6475F2MouseHuIF, FCMAlexa Fluor® 647
PM045Anti-p62 (SQSTM1) (Human) mAbPolyclonalRabbitHu, Mo, Rat,HmWB, IP, IF, IHC
PM066Anti-p62 C-terminal (Human) pAbPolyclonalGuinea PigHu, Mo, Rat, HmWB, IP, IF, IHC
PM066-7Anti-p62 C-terminal pAb-HRP-DirecTPolyclonalGuinea PigHu, Mo, Rat, HmWBHRP
M217-3Anti-Phospho-p62 (SQSTM1) (Ser351) pAb5D5MouseHu,MoWB, IF, IHC
PM074Anti-Phospho-p62 (SQSTM1) (Ser351) pAbPolyclonalRabbitHu,MoWB, IP, IF, IHC
D343-4Anti-Phospho-p62 (SQSTM1) (Ser403) mAb4F6RatHu,MoWB,IHC
D344-3Anti-Phospho-p62 (SQSTM1) (Ser403) mAb4C8RatHu,MoWB,IHC

Blog originally posted by Tomohiro Nakajo on Jan 9, 2018 10:24:11 AM

MBL International products are distributed in the UK and Ireland by Caltag Medsystems. If you have any questions about the products in this post, please contact us at, or call +44 (0)1280 827460.

Mechanisms and detection methods of p62/SQSTM1 and its importance in the autophagy pathway.
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