FUCCI (Fluorescent Ubiquitination-based Cell Cycle Indicator) is a set of fluorescent probes which enables the visualisation of cell cycle progression in living cells. FUCCI utilises the phase-dependent nature of replication licensing factors Cdt1 and Geminin. A fusion protein of a
The Ubiquitin-Proteasome System
The UPS and the autophagic-lysosomal pathway are the two major degradation systems for native & misfolded proteins in eukaryotic cells.