Expressing recombinant antibodies can be done in all kinds of systems. Many different cell lines can be used for expressing antibodies, including mammalian cells like HEK293 or CHO, or even yeast, plant or insect cell lines. Each expression system has
Antibody Basics: Recombinant Antibodies
Antibody development works much like any engineering feat; you get out what you put in. With the shotgun approach to developing polyclonal antibodies, hitting the target usually isn’t too much of a problem, but the immune response can be nonspecific.
Antibody Basics: Isotypes
Antibodies come in several shapes and sizes, leading to a variance in properties and purposes. Below is a broad outline of each of the major isotypes of antibodies and how they are used. Some isotypes can be isolated incredibly easily