When performing staining with MHC tetramers, there may be certain cases where the staining is unclear. An example could be in certain cases where an antigen-specific T cell population of interest has a low frequency. The following tips discussed in
Evaluating the biological activity of a cancer vaccine with the HLA-A*02:01 QuickSwitch™ Quant platform
Principle of the cell-based T2 shift assay and the cell-free QuickSwitch™ Quant assay Comparison of the cell-based T2 shift assay and the cell-free QuickSwitch™ Quant assay Immuno-oncology is a growing field in which different strategies such as immune modulators, vaccines
Non-classical MHC
Non-classical MHC: HLA-E and Qa-1b Human leukocyte antigen E (HLA-E) is a non-classical class I molecule recognised by natural killer (NK) cells and CD8+ T cells. HLA-E is expressed in almost all tissues including lung, liver, skin and placental cells. HLA-E expression
Help! My mouse tetramer stained ALL the CD8+ T cells!
You’re sitting at your flow cytometer, staring at your CD8 x tetramer plot in anticipation of a tiny but meaningful group of dots appearing in the upper right quadrant. You hold your breath, click “acquire,” and watch… Wait, what? What’s