Biotech Support Group bring you two alternate strategies for haemoglobin removal from sample preparations for proteomic & genomic sample seperation and enrichment
Hemoglobind – a binding strategy recommended for applications where haemoglobin is in relatively low abundance ie haemolysed serum.
HemoVoid – a haemoglobin voiding strategy recommended for applications where haemoglobin is in relative high abundance ie erythrocyte lysates or whole blood.
Plus trial kits: Hemoglobind Trial Kit and HemoTrial Kit.
The Hemoglobind™ group of reagents for Haemoglobin Removal and Capture includes:
- HemogloBind™ -Hemoglobin Depletion From Hemolyzed Serum/Plasma
- NuGel-HemogloBind™ – Hemoglobin Capture Reagent From Blood and Hemolyzed Serum
Hemoglobind™ – a solution for Haemoglobin Removal and Capture.
Poly-electrolytes are polymers with repeating units of stationary charges. HemogloBind™ comes from a class of solid-phase, or surface-based, elastomeric poly-electrolytic surfaces that bind proteins through an empirically derived chemistry combining elements of polymer composition, cross-linking architecture and charge properties. As with bio-polymers like DNA and Heparin, governing their reactivity is the spatial presentation of the electrostatic groups along a flexible polymer chain.
Hemoglobind™ has a high degree of specificity for hemoglobin, without cross-reacting with other analytes. It can be used with the following samples to study analytical interferences, enzyme monitoring and blood substitutes:
- Haemolysed serum/plasma
- Whole Blood or Red Cell lysates
- Tissue Homogenates
HemogloBind™ does not cross react with most common serum components, making it an excellent tool in numerous applications. These include analytical protocols where optical interference is problematic, such as bilirubin analysis and bulk serum clarification. Haemoglobin variants, as in thalassemia, bind with differential affinity towards HemogloBind™. For purification and/or analysis of haemoglobin, a modest elevation in pH will facilitate desorption of hemoglobin bound to HemogloBind™.
Product Information
Hemoglobind (5ml) – Product Code: H0145-05
Hemoglobind (15ml) – Product Code: H0145-15
Hemoglobind (50ml) – Product Code: H0145-50
Hemoglobind Trial Kit (5 mL Hemoglobind & 5 preps of NuGel HemogloBind) – Product Code: HB145K-05
Key Reference
Parvathi S. Kumar, et al. Peptide inhibitor of complement C1 modulates acute intravascular hemolysis of mismatched red blood cells in rats. TRANSFUSION Volume 00, May 2016.
NuGel-HemogloBind™ – Hemoglobin Capture Reagent From Blood and Hemolyzed Serum
- Has a high degree of specificity for hemoglobin binding up to 10 mg/ml
- Removes hemoglobin from any species including human, mouse, rat, sheep, bovine, goat, etc
- Removes hemoglobin from organs, tissues.
- Hemoglobin removal from red blood cell lysate for proteomics and biomarker drug discovery
- The flow through fractions(hemoglobin depleted) retain their enzymatic and biological activity
- The flow through fractions(hemoglobin depleted) is compatible with LC-MS, activity based protein profiling and proteomic studies.
- Linearly scaleable up or down. Easy high throughput automation.
NuGel-Hemoglobind™ is reengineered for increased stability. It is based on NuGel™ silica (50 microns in size, 1000Å) covalently bound to elastomeric polyelectrolytes. It binds >95% of hemoglobin from blood.
Product Information
NuGel-HemogloBind™ (25 preps). Product Code: NP-HO-T25
NuGel-HemogloBind™ (50 preps). Product Code: NP-HO-T50
Hemoglobind Trial Kit (5 mL Hemoglobind & 5 preps of NuGel HemogloBind) – Product Code: HB145K-05
HemoVoid – Hemoglobin Depletion Reagent Kit
HemoVoid™ , a silica-based protein enrichment matrix, removes hemoglobin from erythrocyte lysate samples while concentrating low abundance, and/or low molecular weight proteins. The HemoVoid™ protocol uses mild buffers; the protocol conditions are so gentle that native enzyme activity is retained in elution fractions. HemoVoid™ derives from a silica-based library of individual mixed-mode ligand combinations (ionic, hydrophobic, aromatic, polymer). The library was designed to facilitate weak binding of proteins, allowing for rapid elution from the matrix without any foreknowledge of the variety of proteins contained in the starting sample. HemoVoid™ depletes hemoglobin from red cell lysates while improving the resolution of less abundant blood proteins.
- Hemoglobin voids in flow-through >98%, with <30 minute bind/wash/elute protocol
- Hemoglobin removal from red cell lysates for RBC proteomics
- Hemoglobin removal from hemolyzed serum, blood and dried blood spot/blood card
- Enrichment of hemoglobin variants
- Low abundance protein and enzyme enrichment
- Disposable, cost-effective and high-throughput
- Mild elution maintains tertiary structure and simple transfer to secondary analysis
- Removes hemoglobin from species including human, sheep, bovine, goat, etc.
- Removes hemoglobin from organs, tissues.
- The eluted fractions retain their enzymatic and biological activity
- The eluted fraction is compatible with LC-MS, activity based protein profiling and proteomic studies.
Product Information
HemoVoid™ Kit (5 preps) Product Code: HVK-05
HemoVoid™ Kit (10 preps) Product Code: HVK-10
HemoVoid™ Kit (50 preps) Product Code: HVK-50
HemoVoid™ Kit (100 preps) Product Code: HVK-100
Caltag Medsystems are the UK distributor for Biotech Support Group – the manufacturers of Hemoglobind. For further information about their products, please contact us.