When working with primary cells, it is important to remember that they are not cell lines and should be treated with care. At ScienCell, they specialise in primary cell culture and are very familiar with the common problems researchers encounter
Cancer Immunotherapy: A Colourful Past and A Bright Future
cancer_immunotherapy Cancer immunotherapy is one promising cancer treatment option whereby the host’s own immune system is used to treat cancer. The therapy works by either stimulating certain immune activities, or counteracting cancer cell signals that suppress immune
CD138 (Syndecan-1) Detection
CD138 is a member of the trans-membrane heparin sulphate proteoglycan family involved in many cellular functions including cell proliferation, cell migration, cell signalling, cytoskeletal organisation and mediates both cell-cell and cell-extracellular matrix interactions. Additionally, via its heparin sulphate chains, it
Risk-Free™ Immune Checkpoints
ProSci Inc. offers an extensive range of antibodies and supporting tools to research Immune Checkpoints. Read more about their range below, or click a link to jump to a specific section: What Are Immune Checkpoints? What is Risk-Free™? Risk-Free™ Immune
PD-L1 Immune Checkpoint
PD-L1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells. PD-L1 can bind to PD-1, which mediates inhibitory signals from the APC. ProSci Inc. offers an extensive range
Polyfection: Polymer-Based Transfection – The CHAMP Technology
After the development of Lipofection & MagnetofectionTM, OZ Biosciences revolutionises Polyfection with the design & synthesis of a patented CHAMP.
PD-1 Immune Checkpoint
The PD-1 protein is a negative co-stimulatory receptor that can bind two different but related ligands, PD-L1 and PD-L2. Upon binding, signals generated by PD-1 inhibit the activation of the immune response in the absence of “danger signals.” ProSci Inc.
Mechanisms and detection methods of p62/SQSTM1 and its importance in the autophagy pathway.
Mechanisms of Autophagy The ubiquitin-conjugating protein p62/SQSTM1 is thought to be a scaffold protein that interacts with a variety of molecules involved in toll-like receptor (TLR) signaling, such as TRAF6, ERK, and aPKC. Recent evidence suggests that p62 binds directly
Value of qPCR in the Next Generation Sequencing Era
Next generation sequencing (NGS), such as Whole Exome Sequencing (WES) and RNA-Sequencing (RNA-Seq), provides a high throughput approach for DNA sequencing and gene expression analysis. It is rapidly advancing our knowledge on almost all aspects of genetic research and shedding
Primary Neurons vs Neuronal Cell Lines
Neuronal cell lines are commonly used for in vitro neurobiology studies because they are more easily transfected compared to primary neurons and they proliferate, whereas primary neurons do not. Neuronal cell lines can be induced to differentiate into neuron-like cells,