qPCR array provides a quick, powerful and sensitive approach for gene expression profiling. To help our customers acquire accurate and consistent results, we are happy to share some tips for better qPCR. Tip #1. Good primer design is the key
Why is Basal Media and Supplementation Important in Primary Cell Culture?
Choosing which media to use with your primary cells can be daunting. Whether you are replicating a formula from a publication, optimising your own supplemented classical or nutrient media, or purchasing commercially available specialty media, it is important to understand
Six common mistakes when working with primary cells
When working with primary cells, it is important to remember that they are not cell lines and cannot be treated the same. Because ScienCell has extensively worked with primary cells, they are very familiar with the common problems researchers encounter when
Animal Tissue Sourcing Service
Caltag Medsystems has access to fresh and frozen animal tissues that are derived from animals already involved in preclinical testing. Mouse products can be isolated from many different species of mouse including: C57BL/6, BALB/c, C3H, DBA/2, FVB, SJL, 129, DBA/1,
Hepatotoxicity Screening and Mechanistic Testing Applications
Carbohydrate and lipid metabolism HepaRG™ cells display glucose and insulin responsiveness and can be used to investigate carbohydrate homeostasis and lipogenesis. Reactive metabolites The stable and high levels of DMEs in HepaRG™ cells make them ideal for long-term and repeated
Innovative Modes of Culture
BioArtificial Livers (BALs) HepaRG™ cells can be cultured in 3D bioreactors to generate an in-vivo-like assembly of highly polarised cells with high and sustained CYP activities. This makes them a promising cell type for use as liver assist devices or
In-vitro ADME Applications
Drug metabolism and clearance HepaRG™ cells are classified as an “average human hepatocyte population” and express multiple functional Phase 1 and 2 drug metabolising enzyme (DME) activities comparable to levels in cultures of primary human hepatocytes. This makes them a
Silensomes: One single model for DMPK phenotyping studies
Silensomes™ are human pooled liver microsomes in which one specific CYP has been chemically knocked out and are capable of directly quantifying and predicting the contribution of CYP enzymes in drug metabolism. Until now, a battery of in-vitro tests recommended by the regulatory
Request a Cell Culture Booklet
Caltag Medsystems: Your Cell Culture Provider Our Cell Culture portfolio ranges from primary cells, stem cells, cell lines and media to skin, fresh animal brains, healthy & diseased products, angiogenesis models and transfection reagents. We have put together a booklet to provide
Stem Cell Basics
Stem cells, by definition, can undergo infinite number of cell division while remain in an undifferentiated state. However, stem cells can also give rise to differentiated daughter cells that are committed to specialised cell fate in all three primary germ