Diaclone ELISpot: Ex-vivo Analysis of Protein Secretion

Diaclone ELISpot: Ex-vivo Analysis of Protein Secretion

The Enzyme Linked Immunospot (ELISpot) Assay is a versatile and highly sensitive method for ex-vivo analysis of protein secretion post cell stimulation. Virtue of its high sensitivity (1 cell in 100,000) the ELISpot assay enables frequency analysis of activated cell populations or antigen-specific cell responses at the single cell level and therefore can measure the magnitude of specific T-Cell immunity.

ELISpot methodology: when Immunoassay meets bioassay

The ELISpot is a combination of both an immunoassay and bioassay. Living cells are cultured directly into the wells of the ELISpot plate, cells are stimulated and secreted protein captured and detected by sandwich immune-enzyme technology. Diaclone ELISpot assays can detect both secreted cytokines and measure the frequency of single cells that simultaneously produce multiple cytokines or other effector molecules.

ELISpot Applications: measurement of immune outcomes and treatments efficacy

Monitoring the immune responses of patients treated with immunotherapy is essential to evaluate the efficacy of treatment and correlate clinical responses to Cytotoxic T Lymphocyte responses. ELISpot assays are specific, accurate, sensitive precise and robust. This Bioassay is now recognised as the reference method to evaluate vaccine efficacy in clinical trials and has an ever-expanding role in may immunology related research and clinical fields.

Vaccine Development • assessment of vaccine efficacy to induce potent T-cell responses • Antigen-specific cytokine responses • Novel vaccine adjuvants Cancer • Evaluation of the efficacy of novel cancer immunotherapies • Investigation in changes in T-Cell populations during disease progression Allergy • Measurement of Th1 / Th2 cell responses • Evaluation of the efficacy of novel allergen specific immunotherapies Autoimmune Disease • Secretory profile of T-cells under conditions of Autoimmunity Transplantation • Tool for predicting post-transplant rejection • Quantification of frequency and cytokine profile of circulating donor-reactive T-cells

Diaclone ELISpot: respected, reliable & flexible

Respected

  • High sensitivity – detect a single cell out of 100,000
  • Well focused, defined ease to analyse spots
  • Fast procedure post cell stimulation
  • Utilised in research applications through to support of large scale clinical trials

Reliable

  • Accuracy and reliability guaranteed
  • No cross reactivity with other cytokines tested
  • Robust validation to ISO9001

Flexible

  • Enzymatic or fluorescent detection
  • Human Dual ELISpot of Dual fluorospot range – to monitor the production of two cytokines simultaneously in the same well
  • multiple assay formats and sizes available to best suit your needs
  • Flexible supply and custom manufacture and batch allocation to meet you long term study needs

Diaclone ELISpot Formats

Diaclone ELISpot product formats

ELISpot reagents are available in a number of formats from whole pre-coated kits (for easy analysis and increased throughput) to matched Antibody Pairs (a great tool for developing research).

ELISpot Kits: Pre-coated PVDF plate(s), Detection antibody, Alkaline Phosphatase conjugate, BSA and BCIP/NBT ready-to-use substrate buffer.

ELISpot Sets: Capture and Detection antibody, Alkaline Phosphatase conjugate, BSA and BCIP/NBT ready-to-use substrate buffer.

ELISpot Pair: Capture and Detection Antibodies.

Dual ELISpot Sets: Capture and Detection antibodies for two analytes, Alkaline phosphatase conjugate, blocking reagent, Ready-to-use BCIP/NBT substrate buffer, Peroxydase conjugate, BSA, Ready-to-use AEC substrate buffer.

Dual Fluorospot Sets: Capture antibody for two analytes, FITC-conjugated Detection antibody for cytokine 1, anti-FITC antibody green fluorescence conjugate, biotinylated detection antibody for cytokine 2, streptavidin-phycoerythrin conjugate, BSA.

For a full list of ELISpot products, download Diaclone’s catalogue, or search our website.

Need further information on ELISpot?

Diaclone products are distributed in the UK and Ireland by Caltag Medsystems. If you have any questions regarding these product, please feel free to contact us:

Telephone: 0800 279 9113 or +44 (0)1280 827460

Fax: 0800 279 9114 or +44 (0)1280 827466

Sales: sales@caltagmedsystems.co.uk

Technical: techsupport@caltagmedsystems.co.uk

 

Diaclone ELISpot: Ex-vivo Analysis of Protein Secretion
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