QuickSwitch™ – Quickly Create Custom Tetramers

Fast, High-Quality Generation of New Tetramer Specificities

MBL International, distributed by Caltag MedsystemsRapid, high-quality creation of custom class I tetramers is now a reality! Create new specificity tetramers in just a few hours with QuickSwitch™ from MBL International; a proprietary technology for exchanging peptides on an MHC tetramer. The QuickSwitch™ Quant Tetramer Kit can quantify the exchange with the new peptide for epitope discovery and for verification that the resulting new specificity tetramer is suitable for cell staining. This kit is optimised for 10 peptide exchanges and multiple tests per resulting tetramer.

  • Create custom tetramers in 4 hours
  • No UV or Tetramization required
  • Quantify peptide exchange
  • Select ready-to-use tetramer in PE, APC, or BV421
  • Identify antigen-specific T-cells to your antigens of interest

View related scientific posters:

Rapid generation of new specificity MHC tetramers for the detection of antigen-specific T cells using a novel peptide exchange tetramer kit that allows for quantification of peptide exchange

The HLA-A*02:01 Quickswitch™ Quant kit can be used for determining the biological activity of a cancer vaccine

Products

Product DescriptionAlleleConjugates AvailableQuantification Included
QuickSwitch™ Quant HLA-A*02:01 Tetramer KitHLA-A*02:01 PEAPCBV421Yes
QuickSwitch™ HLA-A*02:01 Tetramer KitHLA-A*02:01 PEAPC, BV421No
QuickSwitch™ Quant H-2 Kb Tetramer KitH-2 KbPEBV421Yes
QuickSwitch™ H-2 Kb Tetramer KitH-2 KbPE, BV421No

 

View QuickSwitch™ Products Now

For a limited time, when you purchase a QuickSwitch™ kit for fast, high-quality generation of new tetramer specificities, you will receive 20% OFF ANY MBLI PEPTIDE  included in the order. 

Use promotional code “PEPSWITCH” when ordering

View Stock Peptides

 

Background Information

The immunotherapy field is making great progress in aiding many disease treatments. The hope is that one day, personalised immunotherapy can be used so each patient can use their own immune system to overcome their specific disease affliction. QuickSwitch is a tool for creating custom MHC class I tetramers quickly and easily.

There is currently a great interest in discovering novel peptide sequences from pathogens or tumors (neo-epitopes) that can be used for developing various CD8+ T cell based immunotherapies. The combination of whole-exome and transcriptome sequencing analysis with mass spectrometry now permits rapid identification of a myriad of peptide candidates. As a first screening step, potential binders to MHC molecules can be determined using MHC class I prediction algorithms. However, these algorithms typically generate a large proportion of false binders, therefore requiring experimental validation.

 

Read More on MBL International’s Website

 

QuickSwitch Quant kit

To make this task easier, MBLI have devised a fast, affordable and user-friendly assay (QuickSwitch Quant kit) that can both help determine binding of peptides to MHC class I molecules and generate MHC class I tetramers with these peptides for detection of peptide specific T cells. Peptide exchange does not require any special instrument. Peptides are added to tetramers along with a peptide exchange factor.

Peptide exchange, quantification, cell staining, and flow cytometry analysis can all be performed in one day!

Peptide exchange, quantification, cell staining, and flow cytometry analysis can all be performed in one day!

After a 4 hour incubation, peptide exchange is assessed by a capture assay and quantitated by flow cytometry. New peptide specificity tetramers can then be used directly for staining T cells.

Here is a diagram showing how QuickSwitch Quant assay for peptide exchange works:

QuickSwitch Quantification assay to analyse peptide exchange. Top (no exchange): Exiting peptide not displaced by non-binding peptide. Exiting peptide recognised by FITC-conjugated antibody, resulting in a high MFI(FITC). Bottom (High Exchange): Exiting peptide displaced with high-affinity competing peptide. No anti-exiting peptide antibody binding resulting in low MFI(FITC).

QuickSwitch Quantification assay to analyse peptide exchange. Top (no exchange): Exiting peptide not displaced by non-binding peptide. Exiting peptide recognised by FITC-conjugated antibody, resulting in a high MFI(FITC). Bottom (high exchange): Exiting peptide displaced with high-affinity competing peptide. No anti-exiting peptide antibody binding resulting in low MFI(FITC).

Kits are available with one human allele (HLA-A*02:01) and one mouse allele (H-2 Kb). More alleles will follow in the near future. Quickswitch tetramers are available with three fluorochromes: Phycoerythrin (PE), Allophycocyanin (APC), or Brilliant Violet 421 (BV421).

QuickSwitch kits without Peptide Exchange Quantification

Finally, we also provide Quickswitch kits without the peptide exchange quantitation module. This version may interest researchers who are absolutely sure their peptides are good binders- based on literature, from existing commercial multimers or previous experiments. This provides a significant cost benefit (several tetramers can be made for the price of one) and frees up time (don’t have to wait for custom-made tetramers). Both the Quant kit and Non-Quant kits allow for sensitive peptide sequences to remain confidential.

QuickSwitch tetramers detect similar percentages of low and high affinity CMV responses in PBMCs as classically folded tetramers

Related Product Lines

  • MBLI offers QuickSwitch kits as well as high quality stock and custom peptides. Click here to learn more about their peptide offerings.
  • Over 2000 flow cytometry Antibodies and Reagents. Click here to view their flyer, or click here to view the product range.
  • Classical MHC Tetramers and Monomers. Click here to learn more.

 

Get Your Free Tetramer Staining Guide Here!

 

Email us to request a hard copy.

Caltag Medsystems are the exclusive distributor of MBL International in the UK and Ireland. If you have any questions about any of the products listed above, please contact us on +44 (0)1280 827460, or email techsupport@caltagmedsystems.co.uk.

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