Stress granules inhibit NLRP3 inflammasome activation by sequestering the helicase DDX3X

Inflammasomes are large multimolecular complexes involved in innate immune response and driving cell fate towards cell death, called pyroptosis. Stress granules are aggregates formed during cellular stress with pro-survival role composed of proteins and mRNA molecules. Recently, the lab of Prof. Thirumala-Devi Kanneganti (St. Jude Children’s Research Hospital, Memphis) describes a new role of the stress granules in inhibiting the inflammasome. The stress granule protein DDX3X interacts with NLRP3 to activate the inflammasome. Assembly of stress granules leads to the sequestration of DDX3X, and thereby the inhibition of NLRP3 inflammasome activation. With this dual function, DDX3X regulates a live-or-die cell-fate decisions under stress conditions.

AdipoGen Life Sciences provides the key standard inflammasome antibodies used in this study:

– anti-Caspase-1 (p20) (mouse), mAb (Casper-1) (Prod. No. AG-20B-0042)

– anti-NLRP3/NALP3, mAb (Cryo-2) (Prod. No. AG-20B-0014)

– anti-Asc, pAb (AL177) (Prod. No. AG-25B-0006)

Other reagents in the inflammasome field are also available from AdipoGen Life Sciences:

– anti-Caspase-4 /11 (p20), mAb (Flamy-1) (Prod. No. AG-20B-0060)

– anti-NLRP6/NALP6 (human), mAb (Clint-1) (Prod. No. AG-20B-0046)

– MCC950 . sodium salt [NLRP3 Inhibitor] (Prod. No. AG-CR1-3615)

– Monosodium urate (ready-to-use) [NLRP3 Activator] (Prod. No. AG-CR1-3951)

LIT: DDX3X acts as a live-or-die checkpoint in stressed cells by regulating NLRP3 inflammasome: P. Samir, et al.; Nature (Epub ahed of print) (2019)

Stress granules inhibit NLRP3 inflammasome activation by sequestering the helicase DDX3X
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