To support the efforts of researchers worldwide in understanding and combating SARS-CoV-2, ProSci Inc has developed and manufactured COVID-19 (SARS-CoV-2, 2019-nCoV) antibodies and research reagents against the virus, and virus receptor ACE2. Polyclonal antibodies and monoclonal antibodies are available against COVID-19 virus SARS-CoV-2 spike, envelope, and nucleocapsid protein. Continue reading to find out more about the virus, and the COVID-19 research tools available from ProSci, distributed in the UK by Caltag Medsystems.
What is SARS-CoV-2?
SARS-CoV-2, 2019-nCoV or coronavirus-19, is a novel coronavirus (CoV) originating from Wuhan, China that is spreading worldwide with 2050 laboratory-confirmed infections and 56 fatal cases by January 26, 2020. The World Health Organization (WHO) has declared a global health emergency to gather resources and remedy this threat.
2019-nCoV Background
There are four coronavirus genera: alphacoronavirus, betacoronavirus, gammacoronavirus, and deltacoronavirus. SARS-CoV-2 belongs to the Betacoronavirus genus originating from bats. Betacoronaviruses can infect mammals, are zoonotic pathogens and can cause severe respiratory disease in humans. Other viruses in this family are SARS coronavirus and MERS coronavirus. SARS-CoV-2 has approximately 79% sequence identity to SARS-CoV and 50% to MERS-CoV. In addition, homology modelling shows SARS-CoV-2 has a similar receptor-binding domain structure as SARS-CoV which suggests SARS-CoV-2 may use ACE2 as a receptor in humans for infection.
2019-nCoV Structure
The structure of SARS-CoV-2 consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA as seen in the figure below.
Spike protein is heavily glycosylated, utilizes an N-terminal signal sequence to gain access to the ER and mediate attachment to host receptors. It is the largest structure and makes the distinct spikes on the surface of the virus. For most coronaviruses, S protein is cleaved by a host cell furin-like protease into two separate polypeptides S1 and S2.
RNA is the genome of the virus.
Nucleocapsid protein binds to RNA in vitro and is heavily phosphorylated. N proteins binds the viral genome in a beads on a string type conformation. This protein likely helps tether the viral genome to replicase-transcriptase complex (RTC), and subsequently package the encapsulated genome into viral particles.
Envelope protein is found in small quantities in within the virus. It is most likely a transmembrane protein and with ion channel activity. The protein facilitates assembly and release of the virus and has other functions such as ion channel activity. It is not necessary for viral replication but it is for pathogenesis.
Membrane protein is the most abundant structural protein. It does not contain signal sequence and exists as a dimer in the virion. It may have two different conformations to enable it to promote membrane curvature as well as bind to nucleocapsid.
Hemagglutinin-esterase dimer protein is present in a subset of betacoronaviruses. The protein binds sialic acids on surface glycoproteins. The protein activities are thought to enhance S protein-mediated cell entry and virus spread through the mucosa.
ProSci Coronavirus Antibodies and Recombinant Proteins
ProSci COVID-19 (SARS-CoV-2) Virus Reactive Antibodies
| Description | Product Code | Host | Applications | Clonality | Viral target | Blocking Peptide |
|---|---|---|---|---|---|---|
| SARS-CoV-2/SARS-CoV Spike Antibody | PSI-3223 | Rabbit | ELISA, WB | Polyclonal | Spike Protein | PSI-3223P |
| SARS-CoV-2 (COVID-19) Spike | PSI-3525 | Rabbit | ELISA | Polyclonal | Spike Protein | PSI-3525P |
| SARS-CoV-2 (COVID-19) Envelope | PSI-3531 | Rabbit | ELISA | Polyclonal | Envelope Protein | PSI-3531P |
| SARS-CoV-2 (COVID-19) Nucleoprotein [3851] | PSI-35-579 | Mouse | ELISA, IF, WB | Monoclonal | Nucleocapsid Protein | N/A |
| SARS-CoV-2 (COVID-19) Nucleoprotein [3861] | PSI-35-580 | Mouse | ELISA, IF, WB | Monoclonal | Nucleocapsid Protein | N/A |
| SARS-CoV-2 (COVID-19) Nucleoprotein [3862] | PSI-35-710 (coming soon!) | Mouse | ELISA, IF | Monoclonal | Nucleocapsid Protein | N/A |
| SARS-CoV-2 (COVID-19) Nucleoprotein [3863] | PSI-35-711 (coming soon!) | Mouse | ELISA, IF | Monoclonal | Nucleocapsid Protein | N/A |
| SARS-CoV-2 (COVID-19) Nucleoprotein [3864] | PSI-35-712 (coming soon!) | Mouse | ELISA, IF | Monoclonal | Nucleocapsid Protein | N/A |
ProSci SARS-CoV Antibodies
In addition to antibodies specific to the novel COVID-19 (SARS-CoV-2), ProSci has evaluated their existing SARS-CoV antibodies and determined the percentage of identity and homology between the immunogen used to develop these antibodies and the COVID-19 (SARS-CoV-2) sequences available as of February 15, 2020. A higher percentage of identity and homology suggests reactivity to COVID-19 (SARS-CoV-2).
| Description | Product Code | Host | Application | Clonality | Viral target | Identity | Homology | Blocking Peptide |
|---|---|---|---|---|---|---|---|---|
| SARS Spike Antibody | PSI-3221 | Rabbit | ELISA | Polyclonal | Spike Protein | 56% | 69% | PSI-3221P |
| SARS Spike Antibody | PSI-3225 | Rabbit | ELISA | Polyclonal | Spike Protein | 38% | 56% | PSI-3225P |
| SARS Spike Antibody | PSI-3219 | Rabbit | ELISA | Polyclonal | Spike Protein | 14% | 29% | PSI-3219P |
| SARS Matrix Antibody | PSI-3527 | Rabbit | ELISA | Polyclonal | Membrane Protein | 86% | 93% | PSI-3527P |
| SARS Matrix Antibody | PSI-3529 | Rabbit | ELISA | Polyclonal | Membrane Protein | 8% | 93% | PSI-3529P |
| SARS Envelope Antibody | PSI-3533 | Rabbit | ELISA | Polyclonal | Envelope Protein | 82% | 91% | PSI-3533P |
ProSci COVID-19 (SARS-CoV-2) Recombinant Proteins
| Description | Product Code | Source | Fusion Tag |
|---|---|---|---|
| 2019-nCoV Nucleocapsid Recombinant Protein | PSI-97-077 | E. Coli | His Tag |
| 2019-nCoV 3CL-Mpro Recombinant Protein | PSI-97-078 (coming soon!) | E. Coli | His Tag |
| 2019-nCoV S2 Recombinant Protein | PSI-97-079 | HEK293 | His Tag |
| 2019-nCoV S RBD Recombinant Protein | PSI-97-080 (coming soon!) | HEK293 | Fc Tag |
| 2019-nCoV Envelope Recombinant Protein | PSI-97-082 (coming soon!) | E. Coli | His Tag |
| SARS S1 Recombinant Protein | PSI-97-083 (coming soon!) | HEK293 | His Tag |
| SARS S RBD Recombinant Protein | PSI-97-084 (coming soon!) | HEK293 | His Tag |
For COVID-19(SARS-CoV-2) receptor reagents, check out ProSci’s ACE2 products
Caltag are the UK distributor for ProSci Inc. If you have any questions about ProSci’s COVID-19 products, please contact us.
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